Using Meta-Ethnography to Synthesize Research: A Worked Example of the Relations between Personality on Software Team Processes

Context: The increase in the number of qualitative and mixed-methods research published in software engineering has created an opportunity for further knowledge generation through the synthesis of studies with similar aims. This is particularly true in the research on human aspects because the phenomena of interest are often better understood using qualitative research. However, the use of qualitative synthesis methods is not widespread and worked examples of their consistent application in software engineering are needed. Objective: To explore the use of meta-ethnography in the synthesis of empirical studies in software engineering through an example using studies about the relations between personality and software team processes. Methods: We applied the seven phases of meta-ethnography on a set of articles selected from a previously developed systematic review, to assess the appropriateness of meta-ethnography in this domain with respect to ease of use, and usefulness and reliability of results. Results: Common concepts were identified through reading and interpreting the studies. Then, second order translations were built and used to synthesize a model of the relationships between personality and software team processes. Conclusions: Meta-ethnography is adequate in the synthesis of empirical studies even in the context of mixed-methods studies. However, we believe that the method should not be used to synthesize studies that are too disparate to avoid the development of gross generalizations, which tend to be fruitless and are contrary to the central tenets of interpretive research

Double disruption of alpha(2A)- and alpha(2C)-adrenoceptors results in sympathetic hyperactivity and high-bone-mass phenotype

Evidence demonstrates that sympathetic nervous system (SNS) activation causes osteopenia via beta(2)-adrenoceptor (beta(2)-AR) signaling. Here we show that female mice with chronic sympathetic hyperactivity owing to double knockout of adrenoceptors that negatively regulate norepinephrine release, alpha(2A)-AR and alpha(2C)-AR(alpha(2A)/alpha(2C)-ARKO), present an unexpected and generalized phenotype of high bone mass with decreased bone resorption and increased formation. In alpha(2A)/alpha(2C)-ARKO versus wild-type (WT) mice, micro-computed tomographic (mu CT) analysis showed increased, better connected, and more plate-shaped trabeculae in the femur and vertebra and increased cortical thickness in the vertebra, whereas biomechanical analysis showed increased tibial and femoral strength. Tibial mRNA expression of tartrate-resistant acid phosphatase (TRACP) and receptor activator of NF-kappa B (RANK), which are osteoclast-related factors, was lower in knockout (KO) mice. Plasma leptin and brain mRNA levels of cocaine amphetamine-regulated transcript (CART), which are factors that centrally affect bone turnover, and serum levels of estradiol were similar between mice strains. Tibial beta(2)-AR mRNA expression also was similar in KO and WT littermates, whereas alpha(2A)-, alpha(2B)- and alpha(2C)-AR mRNAs were detected in the tibia of WT mice and in osteoblast-like MC3T3-E1 cells. By immunohistochemistry, we detected alpha(2A)-, alpha(2B)-, alpha(2C)- and beta(2)-ARs in osteoblasts, osteoclasts, and chondrocytes of 18.5-day-old mouse fetuses and 35-day-old mice. Finally, we showed that isolated osteoclasts in culture are responsive to the selective alpha(2)-AR agonist clonidine and to the nonspecific alpha-AR antagonist phentolamine. These findings suggest that beta(2)-AR is not the single adrenoceptor involved in bone turnover regulation and show that alpha(2)-AR signaling also may mediate the SNS actions in the skeleton. (c) 2011 American Society for Bone and Mineral Research.FAPESP, BrazilFAPESP[05/59557-8]FAPESP[06/52982-8]FAPESP[08/50059-3]FAPESP[03/07327-3]CAPES, Brazi